Clinical Assessment of Anti-Aging Benefits of a Skincare Formulation – a biomarker-centered approach to antioxidative defense.

Author: Ana Lucia Tabarini Alves Pinheiro; Silas Arandas Monteiro Silva; Letícia Gomes; Carla Monserrat Grecco Lopes; Barbara de Freitas Carli; Vania Renata Gonçalves; Gustavo Facchini; Samara Eberlin.

Published at: January 01, 2024

https://kosmoscience.com/pdf/clinical-assessment-of-anti-aging-benefits-of-a-skincare-formulation-a-biomarker-centered-approach-to-antioxidative-defense.pdf

Skin homeostasis requires a balance between free radical production and natural antioxidant defense. Since this oxidative process is dynamic, rapid restoration mechanisms are necessary to maintain the structural integrity of the skin. This study aimed to clinically evaluate the effects of a skincare formulation (F4565.33367.200.1) on the production of NRF2 (nuclear factor erythroid 2-related factor 2), SOD (superoxide dismutase), CAT (catalase), GPx (glutathione peroxi dase) and pCOL1 (type I procollagen) in skin biopsies by immunofluorescence analysis. F4565.33367.200.1 was composed by ferulic acid, tocoferol, ascorbic acid and extracts of Inga edulis, Theobroma cacau and Casearia sylvestris. During 28 days, the formulation wasappliedtotherightforearmofresearchparticipants (n¼9, 4665y, ethics committee approval n o5.039.071). Left forearm was considered a control site. Skin biopsies (2mm punch), collected on days T0, T7, T14 and T28 of home use, were immunostained with specific antibodies against the biomarkers. Images were obtained and fluorescence intensity was quantified using ImageJ software. The results demonstrated that, in comparison with control site, F4565.33367.200.1 promotes significant increase of pCOL1 (125.6%-T7; 146.5% T14; 129.2% T28), SOD (34.1%-T28), CAT (169.3%-T7; 99.9%-T14; 127.1%-T28) and GPX(66.1%-T7).Theformulation also promoted anincrease of 40.7%inNRF2 inT7, however not significant. Skin biopsies are invaluable tool in dermatology, offering numerous advantages including accurate research in pharmacology. Using this technique, we prove the antioxidant mechanisms of F4565.33367.200.1 increasing the availability of NRF2, SOD, CATand GPx, and consequentlypCOL1. These results make a valuable contribution to proving the benefits of this antiaging formulation, mitigating the daily oxidative damage to which the skin is exposed