Biomechanical Improvement and Dermal Regeneration: The Transformative Potential of a Novel Cosmetic Formulation.

Author: Silas Arandas Monteiro e Silva; Pedro Soldati; Juliana Rodrigues Pinto; Ana Lúcia Tabarini Alves Pinheiro; Adriano Pinheiro; Viviane Cristina Albarici; Mayara Miranda Ferreira; Barbara Freitas Carli; Gustavo Facchini; Samara Eberlin.

Published at: January 01, 2025

Introduction
Skin aging involves intrinsic and extrinsic factors, leading to epidermal thinning, loss of dermal ridges, and attenuation of papillae, which impair epidermal nourishment.1,2 Additionally, collagen and elastin degradation in the dermis, compounded by reduced fibroblast activity, compromises skin structure and resilience.3,4 This study aimed to clinically evaluate the efficacy of the skincare formulation F4565.33896 in reducing signs of skin aging, using biometric and imaging analyses, along with immunofluorescence analysis of skin biopsies to assess biological markers related to aging.

Methods
This study, approved by the Ethics Committee (project 63574322.4.0000.5514), included 33 participants (64±3 years). A formulation (F4565.33896), containing tocopherol, hydrolyzed hyaluronic acid, and plant-derived bioactive, was applied to one forearm and the face for 56 days, with the opposite forearm as a control. Evaluations included cutometric measurements for firmness and elasticity, digital and 3D imaging for wrinkles, texture, and contours, ultrasonography for dermal density (T0, T28, T56), and skin biopsies (T0, T28) for biomarker analysis (type I pro-collagen, elastin, hyaluronic acid).

Results
Treatment with a formulation F4565.33896 significantly improved skin firmness (19.7% at T28, 20.9% at T56) and elasticity (12.7% at T28, 13.4% at T56). Wrinkles and fine lines reduced by up to 13.7% at T28 and 16.2% at T56, while deep wrinkles decreased by up to 6.9% and 8.5%. Facial texture improved by 8.1% at T28 and 9.1% at T56, and sagging reduced with enhanced contour definition (1.1% at T28, 1.4% at T56). Dermal density increased significantly (11.8% at T28, 19.5% at T56). Additionally, marked boosts in type I pro-collagen (191.85%), elastin (283.95%), and hyaluronic acid production (112.58%) was observed at T28.

Discussion and Conclusion
This study, employing advanced evaluation methods, demonstrated that F4565.33896 significantly improved skin firmness and elasticity, reduced wrinkles and sagging, while also increasing dermal density and enhancing the synthesis of type I collagen, elastin, and hyaluronic acid, suggesting extracellular matrix regeneration. These findings highlight the potential of the active ingredients in the formulation to improve the biomechanical properties of the skin and mitigate signs of aging, consistent with the observed clinical results.

Keyword: anti-aging, aging signs evaluation, skin texture