+55 019 3829-3482  |  +55 019 3829-0841

Effects of antioxidants in the protection of infrared radiation-induced matrix metalloproteinase-1 in human dermal fibroblast


Effects of antioxidants in the protection of infrared radiation-induced matrix metalloproteinase-1 in human dermal fibroblast
Publicado por: Samara Eberlin en 21 de Março de 2014

72nd Annual Meeting AAD - American Academy of Dermatology, Denver-USA, Mach 21-25, 2014. Eberlin S, Pereira AFC, Polettini AJ, Weisz LTM, Mendes C, Lage R, Costa A.

Physiological doses of infrared A radiation (IR-A) lead to a disturbance of the dermal extracellular matrix by upregulation of matrix metalloproteinase-1 (MMP1) and decrease antioxidant enzyme activity1. As such, the infrared induced cytotoxicity, DNA damage and oxidative stress2. Even though no specific chemical or physical filters direct against IRA are available, there is an alternative that can provide a sunscreen more broadly and favoring protection against photodamage, using antioxidant ingredients3, such as Thermus Thermophilus Ferment (FThe)4 and Carnosine (FCar)5. In this study, we evaluated the in vitro effects of two formulations containing the antioxidant ingredients FThe and FCar in the prevention of IR-A-induced damage in human dermal fibroblasts (HDF) through MMP-1 production. HDF were incubated with non-cytotoxic concentrations of FCar and FThe (0.316, 0.100 and 0.0316mg/mL). Cells were exposed to a non-cytotoxic dose3 of 360 J/cm2 IR-A radiation, using a Hydrosun 750T IRA device and the irradiance was measured through a Hydrosun HBM1 irradiance measuring device. The levels of MMP-1 were measured after 72 hours in cultures supernatant using a commercially available kit. Our results demonstrated that irradiation with infrared A resulted in a significant (P<0.0001, ANOVA-Tukey) up regulation of MMP-1 production (8.37±0.44ng/mL) 1.7-fold in relation to non-irradiated group (4.91±0.64ng/mL). FCar at 0.0316 mg/mL produced a significant reduction (P<0.01, ANOVA-Tukey) in the release of MMP-1 by human fibroblast (6.48±0.66ng/mL) in relation to IRA group. In more pronounced (P<0.001, ANOVA-Tukey), FThe at 0.100 and 0.0316mg/mL promoted reductions up to 1.35-fold (6.18±0.51ng/mL and 6.29±0.30ng/mL, respectively) in the levels of MMP-1 when compared to IRA group. The formulations containing FCar) and FThe are suitable for protection of IRA-induced MMP-1 upregulation in vitro in human dermal fibroblast cell culture. These effects can be attributed, at least in part, for the known antioxidant activity of these compounds, although the precise mechanisms remain to be clarified.