Gustavo Facchini, Michelle Sabrina da Silva, Ana Lúcia Tabarini Alves Pinheiro, André Schwambach Vieira, Samir Eberlin, Adriano da Silva Pinheiro, Samara Eberlin
Skin photoaging has been attributed to other spectra of solar radiation such as infrared A (IRA). An alternative method to study these effects is the use of skin explants obtained from plastic surgery, which reflects more accurately a real condition. The aim of this study was to investigate the modulation of genes involved with functional alterations promoted by IRA radiation in human skin explants, through large-scale gene expression using mRNA sequencing (RNA-seq - next generation sequencing). The results showed that IRA radiation is able to modulate genes correlated with neoplasms, such as squamous cell neoplasia and Merkel cell carcinoma. Additionally, hyperalgesia, irritant contact dermatitis and psoriasis presented significant enrichment. Important changes are associated with an increase in inflammatory profile, immune response, formation of inflammassome and exacerbation of oxidative stress. In addition, genes that directly impact the formation of stratum corneum and structure of cutaneous epidermis were also compromised. The results obtained reveal different ways to better understand the impact of IRA radiation on human skin, allowing the development of more accurately evaluation methods to check the effectiveness of active ingredients and products to protect skin from deleterious effects of IRA radiation.