Eberlin S, Polettini AJ, Clerici SP, Eberlin S, Pereira AFC, Lage R, Torloni LBO, Costa A.
Evidence suggests that periorbital hyperchromia occurs mainly as a consequence of post-inflammatory hemodynamic congestion producing a typical bruising aspect on the lower eyelids. Local inflammatory process compromises the microvascular network due to the release of vasoactive mediators, such as, nitric oxide (NO), which is synthesized from inducible NO synthase (iNOS) after tissue disturbance. Another common manifestation observed in periorbital area is the presence of fine wrinkles in consequence of connective tissue breakdown by increasing of matrix metalloproteinases (MMPs) after cytokines and eicosanoids stimulation. In this study we evaluated the effects of a cosmetic eye care (EC) on the production of MMP1, MMP9, tissue inhibitor of MMP-1 (TIMP1) and TIMP2, collagen, elastin, transforming growth factor (TGF-?1) and iNOS, using an in vitro model of human fibroblast. UV radiation promoted significant increases in the levels of MMP1, -9 and iNOS, and decreases in collagen, elastin, TGF-?1, TIMP1 and 2 syntheses, compared to control group. Concomitant treatment of cell cultures with EC reduces the levels of MMP1 and -9 up to 65 and 72%, respectively, compared to UV-stressed group. Increased levels of iNOS were also prevented by EC treatment, reaching undetectable levels similar to baseline control. EC treatment also increases elastin, TGF-?1 and TIMP1 release in relation to UV-stressed group (98, 6 and 75 %, respectively). Although the precise mechanisms related to EC remain to be clarified, our results indicate that the reduction of iNOS levels against deleterious UV-radiation may be considered as an integral approach to preserve the integrity of vascular endothelium, providing adequate cutaneous circulation, which is essential for maintaining healthy skin function and it has a strong relationship with skin color. Furthermore, our results indicate a putative effect of EC on extracellular matrix arrangement, strengthening its use in aesthetic eye care.