Costa A, Eberlin S, Polettini AJ, Jorge ACF, Eberlin S, Pereira AFC, Cascais LC, Dolis E, Frazilho M, Lage R, Torloni LBO
Rosacea is a chronic inflammatory dermatosis characterized by flushing, permanent erythema, telangiectasia, papules and pustules, on the central area of the face that affects middle-aged patients. Its underlying pathophysiological mechanisms are not completely understood, although the disturbances observed are related to vascular reactivity and inflammatory response. Local production of inflammatory mediators, such as, prostaglandins (PG) and histamine (HI) lead to vasodilation and increased vascular permeability due to additional release of VEGF (vascular endothelial growth factor) and inducible nitric oxide synthase (iNOS). In this study we evaluated the effects of an active ingredient (AI) on the production of PGE2, HI, VEGF and iNOS, using an in vitro model of human keratinocytes. Incubation of cell cultures with IL-1? promoted significant increases in the synthesis of HI, PGE2, VEGF and iNOS, compared to control group. Concomitant treatment of cell cultures with AI prevented the increase of all evaluated mediators. In relation to HI and PGE2, AI was able to reduce the levels up to 24,8 and 47%, respectively, compared to IL-1?-stressed group. Increased levels of VEGF and iNOS was also prevented, reaching 51,2 and 100%, respectively, in relation to IL-1?-stressed group. Flushing is one of the most evident signs in Rosacea and increased blood flow in the superficial dermis is the main factor responsible for this phenomenon. In this work, it was demonstrated that AI exerts vasoprotective and anti-inflammatory effects, suggesting a possible application in skin care products formulations to contribute to the amelioration of Rosacea symptoms.