Obesity is a major health problem in the world, due to high prevalence and morbidity. The disposal of fat in the central region is determined by abdominal circumference and features visceral obesity, which is associated with metabolic disorders and can evolve in cardiovascular or cerebrovascular events. Low frequency laser diode therapy is a noninvasive procedure that can be used to reduce body fat through specific wavelength for fat cell, preserving adjacent structures. The aim of this study was to evaluate the reduction of abdominal fat in volunteers undergoing low frequency laser diode therapy. There was a reduction in abdominal circumferences in the evaluated areas, weight and BMI. In the analysis of data from ultrasound (gray-scale), there was a significant reduction of dermis and hypodermis thickness, in relation to D0. We also observed a significant increase in the thickness of the epidermis and Grenz zone in histological sections of biopsies obtained at D0 and D31. Therapy showed a slight decrease in HDL and increased LDL in the analyzed period. There were no reports of adverse events. Low frequency laser diode therapy showed significant results in the reduction of body fat and abdominal circumference measurements. The result shows the ability of laser therapy to assist in the abdominal fat loss and help reduce morbidity and mortality related to central obesity.