Ayres E, Costa A, Clerici SP, Eberlin S. Hyperpigmentation is a commom dermatologic condition, often associated with sun light exposition, and a relatively difficult condition to treat. Pycnogenol, an extract from French maritime pine bark with antioxidant and anti-inflammatory effect, has showed the ability to inhibit tyrosinase activity and melanin biosynthesis in previous studies. Evaluate the skin lightening properties of pycnogenol using an ex vivo model of skin culture after ultraviolet A (UVA)/B (UVB), infrared-A (IR-A) and visible light (VL) irradiation. Histological evaluation demonstrates increase in melanin pigmentation in all fragments irradiated, compared with control fragment. Otherwise, the fragments treated with pycnogenol demonstrate a clear decrease in melanin density after irradiation. This study demonstrates a decrease in melanin deposition in cell culture treated with pycnogenol, after UVA, UVB, IV-A and visible light irradiation and proved it skin lightening properties, suggesting its use in the treatment of cutaneous hyperpigmentation.